Editing Q-94-109A (section)

== Methodology ==
The investigation detailed in Q-94-109A was directed by the [[Naval Research Laboratory]] with a scientific goal to determine the causes of in vivo [[nerve regeneration|neuronal repair]] failure at dendritic termini and to classify the mechanisms and conditions that could allow for proper regeneration.<ref name="Q-94-109A"/>

=== Sample collection ===
Over a span from 19 July 1994 to 25 September 1996, 275 individual aspirative samples were meticulously extracted from specific regions on the right upper appendage of JR. This process utilized pressurized sticks (16mm x 4mm, 0.042 μPsc) inside a [[Pressurized Clean Sphere|C/Sphere]].<ref name="Q-94-109A"/> The samples were taken near analogs of human musculature and neural structures, requiring the [[Dan Burisch|Principal Investigator]] to enter the sphere, an environment declared as an "[[Extraterrestrial Close Encounter]] (E.C.E.), Class IV.c".<ref name="Q-94-109A"/><ref name = "cosmoquest">Burisch Interview mentions PX - Cosmoquest Forum. https://forum.cosmoquest.org/forum/the-proving-grounds/against-the-mainstream/8164-burisch-interview-mentions-px/page2</ref>

=== Sample stabilization and cell culture ===
Post-collection, samples were imaged, labeled, and transferred into [[cell culture]] conditions for stabilization and propagation.<ref name="Q-94-109A"/> Teams led by [[Jonathan Fisher (Naval Research Laboratory)|Captain Jonathan Fisher]] employed [[Cell-Tissue-Culture|CTC]] techniques to subdivide each aspirate (labeled K-24-1 to K-24-100) and propagate them on specialized media.<ref name="Q-94-109A"/><ref name="crain_document"/> Most samples thrived, except for a few (e.g., K-24-16, K-24-36, K-24-81) where heightened osmotic pressure led to cellular death.<ref name="Q-94-109A"/>

The culturing process used a combination of pressurized [[Dictyostelium]]-desoxycholate agar media with a racemic mixture of 10–100% [[glucose]]-[[acetate]] medium, achieving optimal growth rates of approximately 1.000 generations per 26.302 days under strict contamination protocols.<ref name="Q-94-109A"/>

=== Analysis protocols ===
Cells derived from these cultures were prepared for viewing and further analysis following the "E.B.E.-Cross-Contamination and Viewing Protocols".<ref name="Q-94-109A"/> Detailed cellular subfraction analysis, ambient biochemistry, and subfraction biochemistry were conducted as referenced in companion documents Q-94-109B through Q-94-109D.<ref name="Q-94-109A"/>